International Journal of Infertility & Fetal Medicine

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2012 | September-December | Volume 3 | Issue 3

EDITORIAL

Editorial

[Year:2012] [Month:September-December] [Volume:3] [Number:3] [Pages:1] [Pages No:0 - 0]

PDF  |  DOI: 10.5005/ijifm-3-3-iv  |  Open Access |  How to cite  | 

RESEARCH ARTICLE

Alaaeldin Gamal Fayez, Amr Saad El-Sayed, Mohamed Ali El-Desouky, Waheba Ahmed Zarouk, Alaa Khalil Kamel, Ibrahim Mohamed Fahmi, Mona Omar El-Ruby

Molecular Characterization of Some Genetic Factors Controlling Spermatogenesis in Egyptian Patients with Male Infertility

[Year:2012] [Month:September-December] [Volume:3] [Number:3] [Pages:9] [Pages No:69 - 77]

PDF  |  DOI: 10.5005/jp-journals-10016-1045  |  Open Access |  How to cite  | 

Abstract

How to cite this article

Fayez AG, El-Sayed AS, El-Desouky MA, Zarouk WA, Kamel AK, Fahmi IM, El-Ruby MO. Molecular Characterization of Some Genetic Factors Controlling Spermatogenesis in Egyptian Patients with Male Infertility. Int J Infertility Fetal Med 2012;3(3):69-77.

RESEARCH ARTICLE

MS Srinivas, Vickram Sundaram, M Ramesh Pathy, TB Sridharan

A Comparative Study of Distribution of Protein and Cholesterol in Various Fractions of Human Semen from Infertile and Fertile Subjects

[Year:2012] [Month:September-December] [Volume:3] [Number:3] [Pages:5] [Pages No:78 - 82]

PDF  |  DOI: 10.5005/jp-journals-10016-1046  |  Open Access |  How to cite  | 

Abstract

Aim

To elucidate the concentration of the protein and cholesterol in different fractions of human semen from different infertile categories and comparing them with the fertile group.

Materials and methods

The human semen was collected from different infertile categories including oligoasthenospermia, asthenospermia, azoospermia, normospermia, oligospermia and fertile group. Immediately after collection, the semen analysis was done as per WHO standard protocols. After that, the semen was centrifuged to get the different fractions. Four main fractions were obtained, (1) spermatozoa, (2) debris or material that precipitates at 12 K rpm for 10 minutes, (3) prostasomes which was precipitated at 20K rpm for 120 minutes, (4) seminal plasma. The protein concentration was done by Lowry's method and cholesterol was estimated by diagnostic kit.

Results

Sodium dodecyl sulfate—polyacrylamide gel electrophoresis (SDS PAGE) was run for all the categories of semen samples for their seminal plasma and the fertility associated protein was identified. A significant difference was found in the concentration of proteins in all subfractions when compared between control and infertile categories. Almost 86% of the protein was recovered from soluble fraction. In case of azoospermia, the protein content was very low when compared with fertile group. Seminal plasma proteins were visualized by silver staining. The molecular weight of the protein bands were ranging from 6.5 to 205 kDa. The band with molecular weight around 55 kDa was found to be missing in case of oligoasthenospermia. This particular protein is said to be fertility associated protein. The content of cholesterol for different subfraction of the human semen samples from infertile and fertile samples was compared. A wide range of cholesterol was recovered from prostasomes, that too purified.

Conclusion

A thrive study have to be done in all the subfractions of the semen irrespective of the category of samples to know the exact function of the each subfractions in terms of protein and cholesterol distribution.

How to cite this article

Sundaram V, Srinivas MS, Rao KA, Pathy MR, Sridharan TB. A Comparative Study of Distribution of Protein and Cholesterol in Various Fractions of Human Semen from Infertile and Fertile Subjects. Int J Infertility Fetal Med 2012;3(3):78-82.

RESEARCH ARTICLE

Rutvij Jay Dalal, Akansha Mishra

The Correlation between Follicular Fluid Antimullerian Hormone Levels and Fertilization and Embryo Quality in ART Cycles

[Year:2012] [Month:September-December] [Volume:3] [Number:3] [Pages:4] [Pages No:83 - 86]

PDF  |  DOI: 10.5005/jp-journals-10016-1047  |  Open Access |  How to cite  | 

Abstract

Background

Determination of oocyte and embryo quality are one of the most important goals in in vitro fertilization (IVF). Antimullerian hormone (AMH) is secreted by the ovarian granulosa cells into blood flow and follicular fluid. Follicular fluid (FF) AMH level is probably a marker of activity of granulosa cells.

Objective

To evaluate whether high level of FF AMH level is related to success of fertilization and better embryo quality.

Materials and methods

Sixty-two women, whose FF sample was obtained from a single follicle in each patient, underwent IVF with GnRH-agonist long protocol. Based on oocyte fertilization, the patients were divided into fertilized group (n = 42) and nonfertilized group (n = 20). FF AMH levels were measured in both groups and the quality of embryos was determined in fertilized group.

Results

Median of FF AMH level in fertilized group was higher than that in nonfertilized group (5.7 vs 2.7 ng/ml) and a statistically significant difference was observed between the two groups. There was a significant difference between FF AMH level and scores of embryos (p < 0.001). The medians levels of FF AMH were 6.7 ng/ml in good quality embryos and 3.80 ng/ml in fair quality embryos.

Conclusion

Our results indicate that FF AMH level has positive correlation with fertilization and embryo quality; therefore, it can be considered as a marker of IVF outcome.

How to cite this article

Dalal RJ, Mishra A. The Correlation between Follicular Fluid Antimullerian Hormone Levels and Fertilization and Embryo Quality in ART Cycles. Int J Infertility Fetal Med 2012;3(3):83-86.

RESEARCH ARTICLE

Neha Gami, Seema Singhal

An Analysis of Factors Affecting the Duration of Latency Period and Its Impact on Neonatal Outcome in Patients with PPROM

[Year:2012] [Month:September-December] [Volume:3] [Number:3] [Pages:5] [Pages No:87 - 91]

PDF  |  DOI: 10.5005/jp-journals-10016-1048  |  Open Access |  How to cite  | 

Abstract

Introduction

Preterm premature rupture of membranes (PPROM) complicates approximately 3% of all births, but accounts for 30% of neonatal morbidity and mortality among premature gestations. Prediction of latency period for women with PPROM is imprecise and therefore consulting women with PPROM about their predicted latency period is a difficult task. The studies are limited, thus more information is required to support clinical decisions and to provide prognostic information in cases of expectant management following PPROM.

Materials and methods

We conducted a prospective observational study of women with singleton pregnancies presenting with rupture of membranes. A total of 120 women presenting with PPROM from 26 to 36 weeks with rupture of membrane were included in this study.

Results

Advanced maternal age >30 years was found to be associated with prolongation of latency period (p = 0.000). Nulliparity was found to be associated with shortening of latency period (p = 0.012). An inverse association between gestational age at the time of presentation and latency period was established. The average gain in duration of latency period by not doing a digital examination was found to be statistically significant (p = 0.000). Gestational age and duration of latency period were found to be the important predictors of neonatal outcome.

Conclusion

In the current study, several predictive factors were identified which affect the duration of the latency period in cases of PPROM. This information may assist clinician in risk stratification and in providing consultation regarding the natural course of expectant management for women presenting with PPROM.

How to cite this article

Singhal S, Puri M, Gami N. An Analysis of Factors Affecting the Duration of Latency Period and Its Impact on Neonatal Outcome in Patients with PPROM. Int J Infertility Fetal Med 2012;3(3):87-91.

RESEARCH ARTICLE

MS Srinivas, G Ashwini Sidhmalswamy, K Dipika, K Anu, D Mekhala

Comparing the Efficacy of Urinary hCG vs Recombinant hCG for Final Maturation of Oocyte in GnRH Antagonist IVF/ICSI Cycle

[Year:2012] [Month:September-December] [Volume:3] [Number:3] [Pages:5] [Pages No:92 - 96]

PDF  |  DOI: 10.5005/jp-journals-10016-1049  |  Open Access |  How to cite  | 

Abstract

Aims and objectives

To compare the efficacy of urinary human chorionic gonadotropin (uhCG) vs recombinant human chorionic gonadotropin (rhCG) for triggering ovulation in gonadotropinreleasing hormone (GnRH) antagonist in vitro fertilization (IVF) cycle.

Materials and methods

Two hundred patients who underwent GnRH antagonist IVF/ICSI cycles were analyzed, 100 of them received recombinant hCG 250 μg (injection overtrelle) and another group of 100 received urinary hCG (injection ovutrig) either 5000 IU or 10,000 IU for final oocyte maturation and ovulation trigger. The primary outcome measured was total oocytes retrieved and the secondary outcome measured 09 was the maturity rate fertilization rate, cleavage rate and clinical pregnancy rates were also compared.

Results

Age, day 2 follicle stimulating hormone (FSH) and total dose of rFSH were comparable between the groups. There were no significant differences between the groups in terms of the mean number of oocytes retrieved per follicle (uhCG; 12.5, rhCG; 12.09, p-value-0.6698). Similarly, there was statistically no difference in any of the other parameters studied between the two groups. That is number of mature oocytes, number of fertilized oocytes and number of cleaved oocytes, as the p-value was not significant. The clinical pregnancy rate was somewhat higher in the uhCG group but was not statistically significant. Both treatments were well tolerated and there was no significant side effects for either drug.

Conclusion

There is no difference in clinical outcomes between urinary and recombinant hCG for induction of final oocyte maturation. Additional factors, including the cost, drug availability and ease of administration, should be considered, when choosing gonadotropin type.

How to cite this article

Sidhmalswamy GA, Srinivas MS, Dipika K, Anu K, Rao KA, Mekhala D. Comparing the Efficacy of Urinary hCG vs Recombinant hCG for Final Maturation of Oocyte in GnRH Antagonist IVF/ICSI Cycle. Int J Infertility Fetal Med 2012;3(3):92-96.

CASE REPORT

Tejshree Anurag Singh, Abha Majumdar

Successful Obstetrical Management of Over 100-day Interval between the First and Second Twin Delivery in an Infertility-Treated Patient: Counseling and Management Approach to Extreme Asynchronous Twin

[Year:2012] [Month:September-December] [Volume:3] [Number:3] [Pages:5] [Pages No:97 - 101]

PDF  |  DOI: 10.5005/jp-journals-10016-1050  |  Open Access |  How to cite  | 

Abstract

Background

This report describes a patient counseling approach, conservative and surgical management (cervical cerclage) of a dichorionic-diamniotic twin pregnancy, where delivery of the second twin followed the delivery of the first by 104 days.

Case presentation

A 35-year-old Indian lady, with 10 years of primary infertility, conceived twins, following ovulation induction with gonadotropin therapy. At 19 weeks and 4 days gestation, she aborted a female fetus of 410 gm. Tocolytic therapy was initiated; the placenta remained in situ and the umbilical cord of twin A was ligated high and McDonald's cerclage was placed and prophylactic antibiotics initiated. But since the patient's uterus continued to be irritable and the cerclage appeared to be under tension, at 20 weeks 6 days the stitch was taken out and all conservative treatment stopped for 24 hours, but reinitiated after this period, since the uterus remained quiescent. After 3 weeks of uterine inactivity, at 23 weeks 6 days gestation, McDonald's suture was reapplied following which conservative management was offered after counseling for possible risks associated with maternal sepsis, coagulopathy, need for extended hospitalization and potential for hysterectomy. Reassuring fetal status was observed for twin B without evidence of contractions or chorioamnionitis. A viable male infant (1,800 gm) was delivered vaginally at 34 weeks and 4 days gestation.

Conclusion

This report outlines a counseling approach useful for patients with premature delivery of one twin, and presents application of conservative obstetrical management principles for the aftercoming twin, even when delivery interval is extreme.

How to cite this article

Singh TA, Majumdar A. Successful Obstetrical Management of Over 100-day Interval between the First and Second Twin Delivery in an Infertility-Treated Patient: Counseling and Management Approach to Extreme Asynchronous Twin. Int J Infertility Fetal Med 2012;3(3): 97-101.

CASE REPORT

Richa Sharma, Shashikala Nagraj

A Rare Case of Vaginal Dermoid Cyst: A Case Report and Review of Literature

[Year:2012] [Month:September-December] [Volume:3] [Number:3] [Pages:3] [Pages No:102 - 104]

PDF  |  DOI: 10.5005/jp-journals-10016-1051  |  Open Access |  How to cite  | 

Abstract

How to cite this article

Sharma R, Rao KA, Nagraj S. A Rare Case of Vaginal Dermoid Cyst: A Case Report and Review of Literature. Int J Infertility Fetal Med 2012;3(3):102-104.

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