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VOLUME 6 , ISSUE 3 ( September-December, 2015 ) > List of Articles

RESEARCH ARTICLE

Expression Profiling of TGF-β Receptor and its Relation with Endometriosis

Soma Ghoshal, Nisha Rani Agrawal

Citation Information : Ghoshal S, Agrawal NR. Expression Profiling of TGF-β Receptor and its Relation with Endometriosis. Int J Infertil Fetal Med 2015; 6 (3):112-117.

DOI: 10.5005/jp-journals-10016-1112

License: CC BY-NC 4.0

Published Online: 01-08-2012

Copyright Statement:  Copyright © 2015; The Author(s).


Abstract

Study objectives

To measure the transforming growth factorbeta (TGF-β) receptor level in endometriotic tissues in patients selected for surgery in our hospital; and thus to assess its genetic basis in the pathophysiology of endometriosis; and to study its possibility as a potent tissue marker.

Design

Observational study (case control study) involving Genetic Laboratory Study.

Setting

Tertiary Care Institute, Gynecology Department.

Population or sample

Total 100 female patients undergoing surgery in our hospital involved; out of them 50 were cases and 50 controls.

Materials and methods

Fifty cases (having endometriosis) and 50 controls (without endometriosis) were taken. During surgery, excised specimen was examined for presence of any endometriotic tissue. The endometrial tissue samples from suspected area were taken and put immediately in RNA-PCR media and sent to Genetics Laboratory where semi-quantitative RT-PCR analysis of TGF-beta was done using primers designed by Primer Blast software (National Center for Biotechnology Information).

Main outcome measures

The mean TGF-β receptor level was 0.5886 in cases and the mean TGF-β receptor level in controls was 2.076. Both in extrauterine endometriosis and in adenomyosis, TGF-β receptor was downregulated equally in 80% of cases.

Results

Transforming growth factor-beta receptor levels in all types of endometriosis showed a significant down regulation in maximum number of cases.

Conclusion

This downregulation of TGF-β receptor level in the endometriotic tissues not only helps in understanding the pathological basis of endometriosis but most importantly, it can be utilized as genetic basis of therapy in endometriosis in near future.

How to cite this article

Ghoshal S, Agrawal NR, Singh K. Expression Profiling of TGF-β Receptor and its Relation with Endometriosis. Int J Infertil Fetal Med 2015;6(3):112-117.

Consent

Taken from all the 100 patients involved.


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  1. Role of TGF-bs in normal human endometrium and endometriosis. Hum Reprod 2009;00(0):1-9.
  2. Peripheral biomarkers of endometriosis: a systematic review. Hum Reprod Update 2010;00(0):1-24.
  3. The search for genes contributing to endometriosis risk. Hum Reprod Update 2008;14(5):447-457.
  4. Transforming growth factor-pl as a signal for induction of cell death by apoptosis. Br J Cancer 1993;67:531-536.
  5. Polymorphism for Transforming Growth Factor Beta 1-509 (TGF-B1-509): Association with Endometriosis. Biochem Genet 2005;43(5/6):203-210.
  6. Meta-analysis of genome-wide association scans for genetic susceptibility to endometriosis in Japanese population. J Hum Genet 2010;55:816-821.
  7. Endometriosis, retrograde menstruation and peritoneal inflammation in women and in baboons. Hum Reprod Update 2002;8(1):84-88.
  8. Demopoulos. Increased expression of transforming growth factor β isoforms and basic fibroblast growth factor in complex hyperplasia and adenocarcinoma of the endometrium: evidence for paracrine and autocrine action. Cancer Res 1994;54:2347-2358.
  9. TGF-b superfamily expression and actions in the endometrium and placenta. J Reprod 2006;132:217-232.
  10. Signal transduction by the TGF-β superfamily. Sci J 2002;296(5573):1646-1647.
  11. TGF-b signalling from cell membrane to nucleus through SMAD proteins. Nature J 1997;390:465-471.
  12. Induction of endometrial epithelial cell invasion and c-fms expression by transforming growth factor beta. Molecular Hum Reprod 2009;15(10):665-673.
  13. Epigenetics of endometriosis. Molecular Hum Reprod 2009;15(10):587-607.
  14. Transforming Growth Factor-[beta] activity is increased in peritoneal fluid from women with Endometriosis. Obstet Gynecol 1994;83(2):287-292.
  15. Cellular and molecular basis for the regulation of inflammation by TGF-β. J. Biochem 2010;147(6):781-792.
  16. Abrogation of TGF-β signaling in T cells leads to spontaneous T cell differentiation and Autoimmune Disease. Immunity 2000;12:171-181.
  17. Behavior of cytokine levels in serum and peritoneal fluid of women with endometriosis. Gynecol Obstet Invest 2002;54(2): 82-87.
  18. Transforming growth factor β dynamically regulates vascular smooth muscle differentiation in vivo. J Cell Sci 1999;111: 2977-2988.
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