International Journal of Infertility & Fetal Medicine

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VOLUME 5 , ISSUE 3 ( September-December, 2014 ) > List of Articles

RESEARCH ARTICLE

The Effect of Doxycycline on Pus Cells and Oxidative Stress in Male Patients with Leukocytospermia

Richa Aggarwal

Citation Information : Aggarwal R. The Effect of Doxycycline on Pus Cells and Oxidative Stress in Male Patients with Leukocytospermia. Int J Infertil Fetal Med 2014; 5 (3):95-99.

DOI: 10.5005/jp-journals-10016-1089

License: CC BY-NC 4.0

Published Online: 01-12-2014

Copyright Statement:  Copyright © 2014; The Author(s).


Abstract

Objective

The World Health Organization (WHO) has defined leukocytospermia as > 106 WBC/ml of semen. However, the clinical significance of leukocytospermia is currently a subject of controversy. Evidence from several recent studies indicates that leukocytospermia could significantly contribute to oxidative stress and male infertility. Several clinical trials have investigated the efficacy of antibiotic therapy to treat patients with pyospermia in an attempt to improve fertility. Currently, doxycycline is the most common antibiotic used to treat pyospermia though larger trials are needed to demonstrate its efficacy in treating pyospermia.

Materials and methods

Hundred male partners with semen analysis showing the presence of significant leukocytospermia (WBC > 1 × 106/ml), sterile semen culture and satisfying the inclusion and exclusion criteria were enrolled in the study and given doxycycline 100 mg bd for 14 days. Semen analysis for pus cells and oxidative stress (ROS) measurement was done before and after the treatment.

Results

Mean pus cell count before and after treatment with doxycycline was 2.28 ± 1.26 × 106/ml and 1.21 ± 0.58 ×106/mL respectively, the effect being statistically significant (p < 0.05). Resolution of leukocytospermia was seen in 61.4% (54/88) cases after doxycycline treatment. Mean oxidative stress (RLU/ sec/million sperms) before and after doxycycline therapy was 79.72 ± 133.9 and 25.44 ± 47.8, the difference being significant (p < 0.05).

Conclusion

Study results show that treatment with broad spectrum antibiotic like doxycycline leads to significant decrease in the number of pus cells present in semen thereby significantly decreasing the oxidative stress.

How to cite this article

Aggarwal R. the Effect of Doxycycline on Pus Cells and Oxidative Stress in Male Patients with Leukocytospermia. Int J Infertil Fetal Med 2014;5(3):95-99.


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