Aim and background: Chromosomal anomalies frequently occur. Around the world, at least 7.6 million kids are born every year with severe genetic or congenital deformities. One of the biggest problems in contemporary perinatology is the diagnosis of chromosomal abnormalities in fetuses. Trisomies 21, 18, 13, monosomy X, and other sex chromosome aneuploidies are the most prevalent chromosomal abnormalities found in infants. Prenatal diagnostics use a number of approaches to assess a fetus's health and condition. Invasive and non-invasive procedures can be used to diagnose pregnancies. Our objective was to identify indications and complications of amniocentesis. Materials and Methods: A total of 111 pregnant women of gestational age between 16th and 20th weeks who either had abnormal first-trimester scan at 11–14 weeks with positive aneuploidy markers or abnormal mid-trimester anomaly scan with findings suggestive of underlying chromosomal disorders or intrauterine infections were referred from Department of Obstetrics and Gynecology to Department of Radiodiagnosis in Government Medical College, Kozhikode, to undergo amniocentesis to determine the fetal chromosomal structure, numerical aberrations, or translocations. Results: The most common indication for amniocentesis was absent/hypoplastic nasal bone (78%), followed by the combination of absent/hypoplastic nasal bone with increased nuchal fold thickness (7%), smaller percentages including fetal ventriculomegaly (2%), combination of absent/hypoplastic nasal bone with short femur(1%). The complication rate was very minimal; only one patient had a bloody tap. None of the patients had any other complications, such as miscarriages/bleeding per vagina. Mild pain in the abdomen was experienced by a few patients. Conclusion: The most common indication for amniocentesis was absent/hypoplastic nasal bone, followed by a combination of absent/hypoplastic nasal bone with increased nuchal fold thickness. The complications were very minimal. Clinical significance: Amniocentesis is a safe procedure with minimal to no complications. Hence, it can be advised to patients with a positive screening test.