Aims and background: Human immunodeficiency viruses (HIV) have emerged as a widespread crisis, posing a substantial obstacle to both development and public health. Serodiscordant couples of reproductive age encounter an even greater challenge, requiring comprehensive, and accurate information on cohabitation dynamics and measures to safeguard HIV-negative partners from infection. Simultaneously, they maintain equal reproductive rights. The objective of this study is to describe the understanding and behaviors related to HIV and reproductive health among serodiscordant couples receiving care at the HIV-integrated center of Dr Cipto Mangunkusumo National General Hospital in Jakarta, Indonesia. Materials and methods: This study is a cross-sectional descriptive observational study. The study was conducted on 108 respondents. The evaluation of knowledge and behavior levels involved the administration of a validated questionnaire to eligible research participants meeting specific inclusion and exclusion criteria. Results: Among the 108 participants, 59% demonstrated a high degree of HIV knowledge, while 76% exhibited a moderate level of knowledge regarding reproductive health. In terms of behavior, 39.8% consistently used condoms, 41.7% engaged in multiple sexual partnerships, and 10.2% reported engaging in anal intercourse. Notably, 96% adhered to a regular regimen of antiretroviral medications, and none utilized assisted reproductive technology. Additionally, 54.6% of respondents maintained an ongoing reproductive plan, while 39.8% of those with living children had not verified their child's HIV status. Conclusion: The majority of participants demonstrate a solid understanding of HIV, but there is room for enhancement in reproductive health knowledge. There is a need for improved accessibility to information and education on reproductive health, particularly in the context of assisted reproductive technology for HIV serodiscordant couples, in Indonesia.