International Journal of Infertility & Fetal Medicine

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VOLUME 13 , ISSUE 2 ( May-August, 2022 ) > List of Articles


Rapid Molecular Karyotyping for Products of Conception by BACs-on-beads Technology

Preyaporn Onsod, Watcharapa Jaranasaksakul, Prapatsorn Areesirisuk, Rachanee Parinayok, Budsaba Rerkamnuaychoke, Takol Chareonsirisuthigul

Keywords : BACs-on-Beads, Prenatal diagnosis, Products of conception

Citation Information : Onsod P, Jaranasaksakul W, Areesirisuk P, Parinayok R, Rerkamnuaychoke B, Chareonsirisuthigul T. Rapid Molecular Karyotyping for Products of Conception by BACs-on-beads Technology. Int J Infertil Fetal Med 2022; 13 (2):61-66.

DOI: 10.5005/jp-journals-10016-1287

License: CC BY-NC 4.0

Published Online: 16-06-2022

Copyright Statement:  Copyright © 2022; The Author(s).


Aim: Bacterial artificial chromosomes (BACs)-on-beads (BoBs) technology detects aneuploidy, chromosome gains, and losses. The Prenatal™ BoBs detect common aneuploidies and nine microdeletion syndromes. The KaryoLite™ BoBs additionally detect alterations in the p and q arm of all chromosomes. We evaluated BoBs technology for the product of conception (POC) screening. Materials and methods: A total of 265 fresh POC were evaluated by the different BoBs (233 KaryoLite™ BoBs, nine Prenatal™ BoBs, and 23 of both). Results: BoBs detected chromosomal abnormalities in 142 samples (53.58%). The most frequent chromosome abnormality was trisomy 21 (17.61%), followed by monosomy X, trisomy 16, and trisomy 22 (14.08%). Twenty-three samples (13 normal and 10 abnormal), which used both KaryoLite™ and Prenatal™ BoBs kits, were concordant. Moreover, BoBs techniques had no failure rate. Conclusion: BoBs technology is a potentially useful method for diagnosing common aneuploidies and microdeletion syndromes in conception products. This method's advantage over cell culture includes quicker results and a lower failure rate.

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