International Journal of Infertility & Fetal Medicine

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VOLUME 12 , ISSUE 1 ( January-April, 2021 ) > List of Articles


A Retrospective Analysis of Outcome of Vitrified vs Fresh Oocytes among Donor and Patient Cohorts

Rutvij Jay Dalal, Akanksha P Mishra, Divya Rani

Citation Information : Dalal RJ, Mishra AP, Rani D. A Retrospective Analysis of Outcome of Vitrified vs Fresh Oocytes among Donor and Patient Cohorts. Int J Infertil Fetal Med 2021; 12 (1):1-6.

DOI: 10.5005/jp-journals-10016-1210

License: CC BY-NC 4.0

Published Online: 01-04-2021

Copyright Statement:  Copyright © 2021; The Author(s).


Background: Oocyte cryopreservation has been an enigma for many years. It has not been easy to freeze the largest cell of the body owing to its large water content and low surface-to-volume ratio. The recovery of cryopreserved oocytes, for many years, remained dismal at best due to ice crystal formation and insufficient cryoprotectant permeation. In recent years, particularly after the advent of vitrification, a much more successful cryopreservation recovery has been achieved, and oocyte cryopreservation has now become a mainstream treatment procedure in artificial reproduction technology (ART). Aim and objective: Our study aims to retrospectively analyze oocyte cryopreservation data collected over a period of 5 years, between two centers, with a standardized vitrification-warming technique. The parameters taken into consideration were oocyte survival, fertilization, blastocysts formation, implantation rate, and live birth rate. Result: We have observed no significant difference in fresh vs frozen oocytes in the donor as well as patient cohorts in terms of implantation rates and live birth outcomes. Our data have been very encouraging, so that we can offer oocyte cryopreservation to the desiring women seeking this technology, and have opened up a plethora of opportunities with the successful application of oocyte vitrification. Conclusion: We are attempting a further, more elaborate study with much bigger sample size and many more individual criteria to follow-up the success of this technique more closely.

  1. Edgar DH, Gook DA. A critical appraisal of cryopreservation (slow cooling versus vitrification) of human oocytes and embryos. Hum Reprod Update 2012;18(5):536–554. DOI: 10.1093/humupd/dms016.
  2. Kuwayama M, Vajta G, Kato O, et al. Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online 2005;11(3):300–308. DOI: 10.1016/S1472-6483(10)60837-1.
  3. Chen C. Pregnancy after human oocyte cryopreservation. Lancet 1986;1(8486):884–886. DOI: 10.1016/S0140-6736(86)90989-X.
  4. Leibo SP, Songsasen N. Cryopreservation of gametes and embryos of non-domestic species. Theriogenology 2002;57(1):303–326. DOI: 10.1016/S0093-691X(01)00673-2.
  5. Kopeika J, Thornhill A, Khalaf Y. The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence. Hum Reprod Update 2015;21(2):209–227. DOI: 10.1093/humupd/dmu063.
  6. Simopoulou M, Asimakopoulos B, Bakas P, et al. Oocyte and embryo vitrification in the IVF laboratory: a comprehensive review. Folia Med (Plovdiv) 2014;56(3):161–169. DOI: 10.2478/folmed-2014-0023.
  7. Porcu E. Freezing of oocytes. Curr Opin Obstet Gynecol 1999;11(3):297–300. DOI: 10.1097/00001703-199906000-00011.
  8. Donnez J, Dolmans MM. Fertility preservation in women. Nat Rev Endocrinol 2013;9(12):735–749. DOI: 10.1038/nrendo.2013.205.
  9. Gilmore JA, Liu J, Gao DY, et al. Determination of optimal cryoprotectants and procedures for their addition and removal from human spermatozoa. Hum Reprod 1997;12(1):112–118. DOI: 10.1093/humrep/12.1.112.
  10. Jones A, Van Blerkom J, Davis P, et al. Cryopreservation of metaphase II human oocytes effects mitochondrial membrane potential: implications for developmental competence. Hum Reprod 2004;19(8):1861–1866. DOI: 10.1093/humrep/deh313.
  11. Meryman HT. Cryoprotective agents. Cryobiology 1971;8(2):173–183. DOI: 10.1016/0011-2240(71)90024-1.
  12. Nottola SA, Albani E, Coticchio G, et al. Freeze/thaw stress induces organelle remodeling and membrane recycling in cryopreserved human mature oocytes. J Assist Reprod Genet 2016;33(12):1559–1570. DOI: 10.1007/s10815-016-0798-x.
  13. Beca JP, Lecaros A, González P, et al. Medical, ethical and legal issues in cryopreservation of human embryos. Rev Med Chil 2014;142(7):903–908. DOI: 10.4067/S0034-98872014000700011.
  14. Porcu E, Bazzocchi A, Notarangelo L, et al. Human oocyte cryopreservation in infertility and oncology. Curr Opin Endocrinol Diabetes Obes 2008;15(6):529–535. DOI: 10.1097/MED.0b013e3283199129.
  15. Tao T, Zhang W, Del Valle A. Human oocyte cryopreservation. Curr Opin Obstet Gynecol 2009;21(3):247–252. DOI: 10.1097/GCO.0b013e328329c2d2.
  16. Goldman KN, Grifo JA. Elective oocyte cryopreservation for deferred childbearing. Curr Opin Endocrinol Diabetes Obes 2016;23(6):458–464. DOI: 10.1097/MED.0000000000000291.
  17. Kushnir VA, Darmon SK, Barad DH, et al. New national outcome data on fresh versus cryopreserved donor oocytes. J Ovarian Res 2018;11(1):2. DOI: 10.1186/s13048-017-0378-4.
  18. Mattawanon N, Spencer JB, Schirmer 3rd DA, et al. Fertility preservation options in transgender people: a review. Rev Endocr Metab Disord 2018;19(3):231–242. DOI: 10.1007/s11154-018-9462-3.
  19. Lin PY, Huang CC, Chen HH, et al. Failed sperm retrieval from severely oligospermic or non-obstructive azoospermic patients on oocyte retrieval day: emergent oocyte cryopreservation is a feasible strategy. PLoS ONE 2019;14(11):e0224919. DOI: 10.1371/journal.pone.0224919.
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