International Journal of Infertility & Fetal Medicine

Register      Login

VOLUME 11 , ISSUE 2 ( May-August, 2020 ) > List of Articles

Original Article

Influence of Leukocytospermia on Semen Parameters and the Outcome of Intracytoplasmic Sperm Injection

Hela Hannachi, Hanen Elloumi, Salma Ben Khlifa, Manel Hamdoun, Khadija Kacem, Olfa Bahri

Keywords : Leukocytospermia, Male infertility, Semen parameters,Intracytoplasmic sperm injection

Citation Information : Hannachi H, Elloumi H, Khlifa SB, Hamdoun M, Kacem K, Bahri O. Influence of Leukocytospermia on Semen Parameters and the Outcome of Intracytoplasmic Sperm Injection. Int J Infertil Fetal Med 2020; 11 (2):33-36.

DOI: 10.5005/jp-journals-10016-1202

License: CC BY-NC-ND 4.0

Published Online: 09-03-2021

Copyright Statement:  Copyright © 2020; Jaypee Brothers Medical Publishers (P) Ltd.


Abstract

Background: Leukocytospermia (LCS) is implicated in male infertility since it was found in 30% of the infertile male. However, its influence on semen parameters remains controversial. Aims and objectives: This study aimed to determine the impact of LCS on semen parameters and intracytoplasmic sperm injection (ICSI) results in infertile couples. Materials and methods: A retrospective clinical study was conducted at Aziza Othmana Hospital of Tunis. Sperm parameters from infertile men were studied for volume, motility, vitality, and morphology according to seminal leukocytes. Intracytoplasmic sperm injection results were also compared to those observed in a control group matched by age, ICSI indication, hormonal profile, and endometrium thickness. Results: No statistical difference was observed for all semen characteristics except the volume which was higher for the non-leukospermic group (p = 0.035). Conclusion: Intracytoplasmic sperm injection outcomes were lower in the leukospermic group than the controls but the difference was not statistically significant.


PDF Share
  1. Krause W, Herbstreit F, Slenzka W. Are viral infections the cause of leukocytospermia? Andrologia 2002;34(2):87–90. DOI: 10.1046/j.0303-4569.2001.00484.x.
  2. Chen L, Yu SL, Rajesh H. Is semen polymorphonuclear leucocytes count a good predictor of male genital tract infection? Singapore Med J 2013;54(6):328–331. DOI: 10.11622/smedj.2013126.
  3. Thomas J, Fishel SB, Hall JA, et al. Increased polymorphonuclear granulocytes in seminal plasma in relation to sperm morphology. Hum Reprod Oxf Engl 1997;12(11):2418–2421. DOI: 10.1093/humrep/12.11.2418.
  4. Storey BT. Biochemistry of the induction and prevention of lipoperoxidative damage in human spermatozoa. Mol Hum Reprod 1997;3(3):203–213. DOI: 10.1093/molehr/3.3.203.
  5. Aitken RJ, Gordon E, Harkiss D, et al. Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa. Biol Reprod 1998;59(5):1037–1046. DOI: 10.1095/biolreprod59.5.1037.
  6. Sukcharoen N, Keith J, Irvine DS, et al. Predicting the fertilizing potential of human sperm suspensions in vitro: importance of sperm morphology and leukocyte contamination. Fertil Steril 1995;63(6):1293–1300. DOI: 10.1016/S0015-0282(16)57614-6.
  7. Lackner JE, Märk I, Sator K, et al. Effect of leukocytospermia on fertilization and pregnancy rates of artificial reproductive technologies. Fertil Steril 2008;90(3):869–871. DOI: 10.1016/j.fertnstert.2007.07.1310.
  8. Daya S, Wikland M, Nilsson L, et al. Fertilization and embryo development of oocytes obtained transvaginally under ultrasound guidance. J In Vitro Fert Embryo Transf 1987;4(6):338–342. DOI: 10.1007/BF01555382.
  9. Moubasher A, Sayed H, Mosaad E, et al. Impact of leukocytospermia on sperm dynamic motility parameters, DNA and chromosomal integrity. Cent Eur J Urol 2018;71(4):470–475.
  10. Ricci G, Granzotto M, Luppi S, et al. Effect of seminal leukocytes on in vitro fertilization and intracytoplasmic sperm injection outcomes. Fertil Steril 2015;104(1):87–93. DOI: 10.1016/j.fertnstert.2015.04.007.
  11. Cavagna M, Oliveira JBA, Petersen CG, et al. The influence of leukocytospermia on the outcomes of assisted reproductive technology. Reprod Biol Endocrinol RBE 2012;10(1):44. DOI: 10.1186/1477-7827-10-44.
  12. Villegas J, Schulz M, Vallejos V, et al. Indirect immunofluorescence using monoclonal antibodies for the detection of leukocytospermia: comparison with peroxidase staining. Andrologia 2002;34(2):69–73. DOI: 10.1046/j.0303-4569.2001.00476.x.
  13. Ricci G, Presani G, Guaschino S, et al. Leukocyte detection in human semen using flow cytometry. Hum Reprod Oxf Engl 2000;15(6):1329–1337. DOI: 10.1093/humrep/15.6.1329.
  14. Brunner RJ, Demeter JH, Sindhwani P. Review of guidelines for the evaluation and treatment of leukocytospermia in male infertility. World J Mens Health 2019;37(2):128–137. DOI: 10.5534/wjmh.180078.
  15. Punab M, Lõivukene K, Kermes K, et al. The limit of leucocytospermia from the microbiological viewpoint. Andrologia 2003;35(5):271–278. DOI: 10.1111/j.1439-0272.2003.tb00856.x.
  16. Weidner W, Pilatz A, Diemer T, et al. Male urogenital infections: impact of infection and inflammation on ejaculate parameters. World J Urol 2013;31(4):717–723. DOI: 10.1007/s00345-013-1082-7.
  17. Castellini C, D'Andrea S, Martorella A, et al. Relationship between leukocytospermia, reproductive potential after assisted reproductive technology, and sperm parameters: a systematic review and meta-analysis of case-control studies. Andrology 2020;8(1):125–135. DOI: 10.1111/andr.12662.
  18. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003;79(4):829–843. DOI: 10.1016/S0015-0282(02)04948-8.
  19. Ziyyat A, Barraud-Lange V, Sifer C, et al. Paradoxical increase of sperm motility and seminal carnitine associated with moderate leukocytospermia in infertile patients. Fertil Steril 2008;90(6):2257–2263. DOI: 10.1016/j.fertnstert.2007.10.032.
  20. Lackner JE, Agarwal A, Mahfouz R, et al. The association between leukocytes and sperm quality is concentration dependent. Reprod Biol Endocrinol RBE 2010;8(1):12. DOI: 10.1186/1477-7827-8-12.
  21. Erenpreiss J, Hlevicka S, Zalkalns J, et al. Effect of leukocytospermia on sperm DNA integrity: a negative effect in abnormal semen samples. J Androl 2002;23(5):717–723.
  22. Salumets A, Suikkari AM, Möls T, et al. Influence of oocytes and spermatozoa on early embryonic development. Fertil Steril 2002;78(5):1082–1087. DOI: 10.1016/S0015-0282(02)04215-2.
  23. Agarwal A, Makker K, Sharma R. Clinical relevance of oxidative stress in male factor infertility: an update. Am J Reprod Immunol N Y N 1989 2008;59(1):2–11.
  24. Anderson DJ. Should male infertility patients be tested for leukocytospermia? Fertil Steril 1995;63(2):246–248. DOI: 10.1016/S0015-0282(16)57348-8.
  25. Sanocka D, Kurpisz M. Reactive oxygen species and sperm cells. Reprod Biol Endocrinol RBE 2004;2(1):12. DOI: 10.1186/1477-7827-2-12.
  26. Rossi T, Mazzilli F, Delfino M, et al. Improved human sperm recovery using superoxide dismutase and catalase supplementation in semen cryopreservation procedure. Cell Tissue Bank 2001;2(1):9–13. DOI: 10.1023/A:1011592621487.
  27. Cocuzza M, Sikka SC, Athayde KS, et al. Clinical relevance of oxidative stress and sperm chromatin damage in male infertility: an evidence based analysis. Int Braz J Urol Off J Braz Soc Urol 2007;33(5):603–621. DOI: 10.1590/S1677-55382007000500002.
  28. Berkovitz A, Eltes F, Yaari S, et al. The morphological normalcy of the sperm nucleus and pregnancy rate of intracytoplasmic injection with morphologically selected sperm. Hum Reprod Oxf Engl 2005;20(1):185–190. DOI: 10.1093/humrep/deh545.
  29. Barraud-Lange V, Pont J-C, Pocate K, et al. Seminal leukocytes and clinical outcomes with donor sperm insemination. Fertil Steril 2011;96(6):1320–1324.e1. DOI: 10.1016/j.fertnstert.2011. 08.025.
PDF Share

© Jaypee Brothers Medical Publishers (P) LTD.